Abstract
Objective Constructing the dendritic cell (DC) vaccine loading HPV16L1 antigen, and evaluating the specific humoral and cellular immune responses induced by DC-L1. Methods The mature dendritic cells (DCs) were induced in vitro from bone marrow precursors by co-culturing with cytokines cocktail, and then transduced with a recombinant adenovirus harboring the HPV16L1 gene to obtain the DC-L1 vaccine. C57BL/6 mouse were immunized intramuscularly by single dose of DC-L1 and recombinant adenovirus vaccine Adv-HPV16L1 respectively. The specific humoral and cellular immune responses were determined by quantitative ELISA and ELISPOT at different time points. Results DC-L1 cells exhibited typical morphological features of dendritic cells, and the expression of HPV16L1 can be detected by western blot. Both DC-L1 and Adv-HPV16L1 can induce specific cellular immune response, while with distinct kinetics. Single dose of Adv-HPV16L1 induced responses immediately, and attained the peak level at the third week after immunization, then diminished gradually. While responses induced by DC-L1 elevated slowly, and eventually exceeded that of recombinant adenovirus group at the fifth week of post-immunization. As to humoral immunity, both DC-L1 and Adv-HPV16L1 vaccines can induce specific humoral immune reponses, and with similar kinetics. Conclusion The DC-L1 vaccine was constructed successfully. The DC-L1 vaccine can induce high levels of HPV16L1 specific humoral and cellular immune response. Key words: Dendritic cell; vaccine; Papillomavirus human; major capsid protein
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