Construction and immune evaluation of the dendritic cell vaccine pulsed with HPV16L1

Abstract

Objective Constructing the dendritic cell (DC) vaccine loading HPV16L1 antigen, and evaluating the specific humoral and cellular immune responses induced by DC-L1. Methods The mature dendritic cells (DCs) were induced in vitro from bone marrow precursors by co-culturing with cytokines cocktail, and then transduced with a recombinant adenovirus harboring the HPV16L1 gene to obtain the DC-L1 vaccine. C57BL/6 mouse were immunized intramuscularly by single dose of DC-L1 and recombinant adenovirus vaccine Adv-HPV16L1 respectively. The specific humoral and cellular immune responses were determined by quantitative ELISA and ELISPOT at different time points. Results DC-L1 cells exhibited typical morphological features of dendritic cells, and the expression of HPV16L1 can be detected by western blot. Both DC-L1 and Adv-HPV16L1 can induce specific cellular immune response, while with distinct kinetics. Single dose of Adv-HPV16L1 induced responses immediately, and attained the peak level at the third week after immunization, then diminished gradually. While responses induced by DC-L1 elevated slowly, and eventually exceeded that of recombinant adenovirus group at the fifth week of post-immunization. As to humoral immunity, both DC-L1 and Adv-HPV16L1 vaccines can induce specific humoral immune reponses, and with similar kinetics. Conclusion The DC-L1 vaccine was constructed successfully. The DC-L1 vaccine can induce high levels of HPV16L1 specific humoral and cellular immune response. Key words: Dendritic cell; vaccine; Papillomavirus human; major capsid protein