Abstract
Objective To construct and identify the recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Methods Whole sequence of galectin-1 was obtained from original plasmid double-digested with Kpn I/BamH I enzymes.Then Galectin-1 DNA segments were linked into pShuttle-CMV-EGFP vector to obtain recombinant pShuttle-GFP-Galectin-1 plasmid.Next,recombinant DNA sequences CMV-GFP-Galectin1were double-digested with I-Ceu I/I-Sce I enzymes and constructed into pShuttle-GFP-Galectin-1 vector to establish recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Results Through endonuclease and gene sequencing the target gene was verified to be corrrctly cloned in adenvrius vector.Conclusion The recombinant adenovirus vector pAdxsi-GFP-Galectin-1 was successfully constructed. Key words: Recombinant adenovirus vector; Galectin-1
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