Abstract
Objective To construct recombinant adenovirus carrying the target gene B cell Lymphoma/Leukemia-Ⅺ (bcl-xL).Methods Recombinant adenovirus pAdxsi-GFP-bcl-xL was constructed and infected into human umbilical vein endothelial cells (HUVECs).The expression of bcl-xL in HUVECs after infection was assessed.After oxidative stress treatment,apoptosis rate was evaluated through Annexin V/FITC and PI staining.Results The recombinant adenovirus pAdxsi-GFP-bcl-xL was successfully constructed.The target protein bcl-xL was overexpressed in HUVECs after infection.Under the condition of oxidative stress in vitro,the early apoptosis rate of HUVECs in bcl-xL overexpression group was lower than the H2O2 group [ (3.7 ± 0.2)％ vs (10.3± 0.5)％,P ＜ 0.01 ],as was the mid and late apoptosis rate [ (6.8 ± 1.3 ) ％ vs ( 18.3 ± 2.8) ％,P ＜ 0.05 ].Conclusion The recombinant adenovirus pAdxsi-GFP-bcl-xL has been successfully constructed.bcl-xL overexpression can significantly decrease the apoptosis rate of VECs under the oxidative stress. Key words: B cell Lymphoma/Leukemia-Ⅺ; Adenovirus; Umbilical vein endothelial cells; Oxidative stress
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
