Abstract
We have constructed novel chimeric proteins that consisted of a single domain of protein A and luciferase originated from marine firefly Vargula hilgendorfii (Umi Hotaru in Japanese) with the aim of obtaining a bifunctional immunological tool. D domain gene of protein A was fused to the 5’ terminus of luciferase cDNA with/without a short linker encoding five amino acids. The resulting constructs were transfected and proteins were expressed in cultured COS-1 or CHO cells either transiently or stably, respectively. The properties of the resultant chimeric protein were characterized by luminometry, immunoblot analysis and affinity binding studies. The results show that the dual biological properties of the chimeric protein could be retained after the introduction of the linker between the two moieties.
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