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Abstract
A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide (NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione (GSNO). Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts.
Highlights
Cardiovascular diseases as coronary artery occlusion are a serious threat to human health worldwide [1]
To simulate the function of native blood vessel, we proposed to modify the surface of vascular graft with organoselenium catalyst to generate nitric oxide (NO) stably from endogenous NO donor RSNO
We proposed a novel method of improving the hemocompatibility and bioactivity of artificial blood vessel by loading organoselenium catalyst onto the inner surface of electrospun tubular graft
Summary
Polycaprolactone (PCL, Mw 80 kD) was purchased from Solvay Interox Ltd, UK. Polyethyleneimine (PEI, Mw 25 kD) and polydiallyldimethylammonium chloride (PDDA, Mw 100 200 kD) were obtained from Sigma-Aldrich. 2-(Ncyclohexylamino)-ethanesulfonic acid (CHES), sodium alginate (AlgNa) and glutathione (GSH) were purchased from Beijing Dingguo Biotech Co. Ltd. MTT was obtained from Lianxing Biotechnology Inc. DiI was purchased from Invitrogen. Griess Assay Kit was purchased from Beyotime Institute of Biotechnology
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