Abstract
BackgroundEndometrial carcinoma (EC) is one of the most common gynecological malignant tumors. In this study, we constructed gene co-expression networks to identify key modules and hub genes involved in the pathogenesis of EC.ResultsThe MEturquoise module was found to be significantly related to hypertension and the MEbrown module was significantly related to the history of other malignancies. Functional enrichment analysis showed that the MEturquoise module was associated with the GO biological process terms of transcription from RNA polymerase II promoter, positive regulation of male gonad development, endocardial cushion development, and endothelial cell differentiation. The MEbrown module was associated with GO terms DNA binding, epithelial-to-mesenchymal transition, and transcription from RNA polymerase II promoter. A total of 10 hub genes were identified and compared with the available datasets at transcriptional and translational levels.ConclusionsThe identified ceRNAs may play a critical role in the progression and metastasis of EC and are thus candidate therapeutic targets and potential prognostic biomarkers. The two modules constructed further provide a useful reference that will advance understanding of the mechanisms of tumorigenesis in EC.
Highlights
Endometrial carcinoma (EC) is one of the most common gynecological malignant tumors
The mRNA in competing endogenous RNA (ceRNA) was obtained by intersection of the predicted target gene and the selected differential RNA (Fig. 1d)., The constructed ceRNA network diagram of differentiated RNAs in endometrial adenocarcinoma is WGCNA Twenty-seven cancer samples were randomly selected from the The Cancer Genome Atlas database (TCGA) data for further WGCNA analysis of the 196 differentially expressed RNAs in the ceRNA network
Based on the final scale-free network constructed (Fig. 3c–d), the GeneSignificance(GS) and Module membership (MM) values of the modules were calculated according to combined analysis of modules and trait data along with mining the modules and key genes that play a key role in endometrial adenocarcinoma (Supplementary Material 7)
Summary
Endometrial carcinoma (EC) is one of the most common gynecological malignant tumors. In this study, we constructed gene co-expression networks to identify key modules and hub genes involved in the pathogenesis of EC. Endometrial carcinoma (EC) is one of the most common gynecological tumors worldwide, and its incidence has been on the rise every year in both developed and developing countries [1]. In contrast to other gynecological tumors such as ovarian cancer and cervical cancer that are associated with highly specific landmark molecules such as CA125, HE4, and SCC [6,7,8], no such markers are available for EC, which is a challenge for clinical diagnosis. Dilatation and curettage [15, 16] and hysteroscopy are potentially effective screening methods, but are highly invasive and not recommended. Further effort is needed to identify candidate molecular markers to improve the screening and early diagnosis of EC, as well as to provide insight into the pathogenic mechanism
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