Construction and comparison of Prussian blue analogs nanomaterials with tailorable coordination metal ions as novel adsorbents for efficient structure-selective protein adsorption

Abstract

Efficient and selective purify histidine (His)-rich proteins from complex biological sample for disease initiation and progression diagnostic is crucial, but it is still a challenge. Herein, for the first time, nanomaterials composed of Prussian blue analogues (PBAs) with tailorable coordination metal ions were synthesized and operated as adsorbents for selective separation of His-rich proteins. Benefiting from the strong metal affinity forces between the His residual in protein and the immobilized Cu2+, Ni2+ and Co2+ in CuFe, NiFe and CoFe PBAs, the obtained adsorbents exhibit highly selective adsorption performance for structure-selective proteins toward bovine hemoglobin (BHb). As a results, the maximum adsorption capacities of CuFe, NiFe and CoFe PBAs for BHb are 3365.8 mg g−1, 2421.3 mg g−1 and 1791.2 mg g−1, respectively. Moreover, the high-performance CuFe, NiFe and CoFe PBAs can be easily regenerated and used to effectively separation of BHb from real bovine blood samples, permitting their excellent practicality in the complex biological sample pretreatment, and further expanding the application scope of the traditional classical coordination compound in biochemical analysis fields.