Abstract
Citri Reticulatae Pericarpium (CRP), known as Chenpi (CP) in Chinese, is a medicinal food for health and fitness. In order to find out the characteristic activity chemicals distinguishing various cultivars of CRP and provide a reference for effective development of citrus resources, an “activity fingerprint” of CRP from 21 different cultivars was established based on the evaluation of antitussive and expectorant activities. There were 18 common peaks in the HPLC fingerprint, of which 3 flavonoid glycosides and 14 polymethoxyflavonoids (PMFs) were identified by LC/MS-IT-TOF. Furthermore, five characteristic chemicals were determined and similarity calculation with principal component analysis (PCA) was combined together to compare the similarity and difference among various cultivars. The results showed that some other cultivars were also similar to C. reticulata “Chachi” except for C. reticulata “Tangerina” and C. reticulata “Dahongpao” recorded in Chinese Pharmacopoeia. Most importantly, the peels of C. reticulata “Shiyueju,” C. reticulata “Ponkan,” C. reticulata “Tribute,” and C. reticulata “Bayueju,” traditionally rarely used for medicinal food, were highly similar to that of C. reticulata “Chachi” and rich in bioactive flavonoids, which can be considered the effective medicinal resources of CRP.
Highlights
Citri Reticulatae Pericarpium (CRP), known as Chenpi (CP) in Chinese, the dry mature peel of Citrus reticulata Blanco or its cultivated varieties [1], has been traditionally used as both nutritional food and herbal medicine for clinic treatment of cough with phlegm, vomiting and diarrhea, and stomach ache [2]. ere are four cultivars of CRP officially listed in Chinese Pharmacopoeia (2015 version), such as C. reticulata “Chachi,” C. reticulata “Dahongpao,” C. reticulata “Tangerina,” and C. reticulata “Unshiu.” Among them, the desiccative ripe peel of Citrus reticulata “Chachi,” calledGuang Chenpi (GCP) in Chinese and produced in Xinhui (Guangdong, China), is well known as a traditional genuine herb with high quality
It is a good assumption to evaluate and compare a great number of CRP samples through the High-performance liquid chromatography (HPLC) fingerprint, previous studies mainly focused on a single cultivar of C. reticulata “Chachi” from different places of Guangdong Province or four recorded cultivars [5], while other nonrecorded but common cultivars have not been taken into consideration
Statistical analysis showed significant differences between the control group and treated groups in the incubation period of cough (P < 0.05), bouts of cough within 3 minutes (P < 0.05), and tracheal phenol red excretion (P < 0.05). e test of ammonia water-irritated cough showed that the methanol extract of CRP (MECRP) significantly prolonged the incubation period of cough and decreased the bouts of coughs in mice, possessing a good ability to relieve cough
Summary
Citri Reticulatae Pericarpium (CRP), known as Chenpi (CP) in Chinese, the dry mature peel of Citrus reticulata Blanco or its cultivated varieties [1], has been traditionally used as both nutritional food and herbal medicine for clinic treatment of cough with phlegm, vomiting and diarrhea, and stomach ache [2]. ere are four cultivars of CRP officially listed in Chinese Pharmacopoeia (2015 version), such as C. reticulata “Chachi,” C. reticulata “Dahongpao,” C. reticulata “Tangerina,” and C. reticulata “Unshiu.” Among them, the desiccative ripe peel of Citrus reticulata “Chachi,” calledGuang Chenpi (GCP) in Chinese and produced in Xinhui (Guangdong, China), is well known as a traditional genuine herb with high quality. High-performance liquid chromatography (HPLC) fingerprint analysis has recently been applied as a quality evaluation method with high specificity, stability, Evidence-Based Complementary and Alternative Medicine reproducibility, and integrity because a large number of samples could be well analyzed by describing the similarities and differences through similarity results [3, 4]. It is a good assumption to evaluate and compare a great number of CRP samples through the HPLC fingerprint, previous studies mainly focused on a single cultivar of C. reticulata “Chachi” from different places of Guangdong Province or four recorded cultivars [5], while other nonrecorded but common cultivars have not been taken into consideration. It is more reliable to find out characteristics distinguishing varieties to protect the health of the CRP market when adopting as many different cultivars as possible for quality comparison. The HPLC fingerprint is just a kind of qualitative analysis and the compounds corresponding to its characteristic peak have not been profiled [6], so it is of significance to further study the characteristic components in combination with other identification and quantification techniques
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Evidence-Based Complementary and Alternative Medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
