Abstract
Background: Infection that is related to implanted biomaterials is a serious issue in the clinic. Antimicrobial peptides (AMPs) have been considered as an ideal alternative to traditional antibiotic drugs, for the treatment of infections, while some problems, such as aggregation and protein hydrolysis, are still the dominant concerns that compromise their antimicrobial efficiency in vivo. Methods: In this study, antimicrobial peptides underwent self-assembly on gold substrates, forming good antibacterial surfaces, with stable antibacterial behavior. The antimicrobial ability of AMPs grafted on the surfaces, with or without glycine spaces or a primer layer, was evaluated. Results: Specifically, three Pac-525 derivatives, namely, Ac-CGn-KWRRWVRWI-NH2 (n = 0, 2, or 6) were covalently grafted onto gold substrates via the self-assembling process for inhibiting the growth of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, the alkanethiols HS(CH)10SH were firstly self-assembled into monolayers, as a primer layer (SAM-SH) for the secondary self-assembly of Pac-525 derivatives, to effectively enhance the bactericidal performance of the grafted AMPs. The -(CH)10-S-S-G6Pac derivative was highly effective against S. aureus and E. coli, and reduced the viable amount of E. coli and S. aureus to 0.4% and 33.2%, respectively, after 24 h of contact. In addition, the immobilized AMPs showed good biocompatibility, promoting bone marrow stem cell proliferation. Conclusion: the self-assembled monolayers of the Pac-525 derivatives have great potential as a novel therapeutic method for the treatment of implanted biomaterial infections.
Highlights
Bacterial infection is one of most serious issues that leads to the failure of implanted biomaterials in the clinic
The water contact angle (WCA) measurements showed that the hydrophilicity of the Antimicrobial peptides (AMPs)-functionalized surfaces increased in comparison with the an untreated gold substrate (Au) substrate or primer layer (SAM-SH), demonstrating that the peptides were successfully self-assembled on the substrates
It is noted that the surface hydrophilicity increased slightly in the self-assembled monolayers (SAMs) of the AMP with a longer spacer length, which implied that the spacers might be beneficial for promoting the self-assembly of the Pac-525 peptides
Summary
Bacterial infection is one of most serious issues that leads to the failure of implanted biomaterials (e.g., implants, artificial joints, contact fixators, and compression plates) in the clinic. Approaches that offer stable long-lasting antibacterial capacity, to prevent the formation of bacterial bio-films on the surface of implanted biomaterials, have a huge clinical impact [3,4]. Over the last few decades, many studies that focused on the inhibition of pathogenic bacterial infections have proposed several strategies to enhance the antibacterial performances of biomaterials, including external morphology modification, physical entrapment of antibacterial substances, and surface grafting of antibacterial substances via chemical treatments [5,6,7]. Methods: In this study, antimicrobial peptides underwent self-assembly on gold substrates, forming good antibacterial surfaces, with stable antibacterial behavior. The antimicrobial ability of AMPs grafted on the surfaces, with or without glycine spaces or a primer layer, was evaluated
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
