Abstract
We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy.
Highlights
IntroductionThe lack of a complete genome sequence limits the research available of the wheat genome
Wheat is one of the most important food crops in the world [1]
The transformation efficiencies of the 3-day ligation reactions with 1.5–5.1 × 105/μg DNA were 6.8–9.2 times higher than the efficiency of 1-day ligation (Table 1) and 1.5 to 5 times higher than the 1 × 105/μg DNA of the wheat bacterial artificial chromosome (BAC) library of DV92 constructed by Lijavetzky et al [12]
Summary
The lack of a complete genome sequence limits the research available of the wheat genome Under these circumstances, a large-insert genome library is critical for physical mapping, map-based gene cloning, whole-genome sequencing, Int. J. BAC libraries of small-genome grass species such as rice have been used for comparative mapping and gene cloning in wheat [6,7,8,9]. This strategy could be adventurous because of genome rearrangement and evolution. An improved descending pooling system for BAC library screening is developed and applied
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
