Abstract
This work describes the construction and application of a biomimetic sensor for paracetamol determination in different samples . The sensor was prepared by modifying a glassy carbon electrode surface with a Nafion® membrane doped with FeTPyPz. The best performance of the sensor in 0.1 mol L-1 acetate buffer was at pH 3.6. Under these conditions, an oxidation potential of paracetamol was observed at 445 mV vs. Ag|AgCl. The sensor presented a linear response range between 4.0 and 420 µmol L-1, a sensitivity of 46.015 mA L mol-1 cm-2, quantification and detection limits of 4.0 µmol L-1 and 1.2 µmol L-1, respectively. A detailed investigation about its electrochemical behavior and selectivity was carried out. The results suggested that FeTPyPz presents catalytic properties similar to P450 enzyme for paracetamol oxidation. Finally, the sensor was applied for paracetamol determination in commercial drugs and for the monitoring of its degradation in an electrochemical batch reactor effluent.
Highlights
There are many enzymes in nature that can be mimicked aiming for biomimetic sensor constructions,[1] which the chemistry of the enzymatic catalysis is well known
The results showed that paracetamol irreversibly oxidizes on the electrode surface at potentials higher than 600 mV vs. Ag|AgCl
These results demonstrated the inefficiency of the unmodified glassy carbon (GC) electrode for paracetamol quantification
Summary
There are many enzymes in nature that can be mimicked aiming for biomimetic sensor constructions,[1] which the chemistry of the enzymatic catalysis is well known. In this work we described the use of FeTPyPz complex as a biomimetic catalyst of P450 enzyme and its application in the construction of a sensor for paracetamol determination in pharmaceutical samples taken from an electrochemical batch reactor effluent that was used for drug degradation.
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