Abstract
Natural eucalyptus biomorphic porous carbon (EPC) materials with unidirectional ordered pores have been successfully prepared by carbonization in an inert atmosphere. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) were employed to characterize the phase identification, microstructure and morphology analysis. The carbon materials were used to fabricate electrochemical sensors to detect hydrogen peroxide (H2O2) without any assistance of enzymes because of their satisfying electrocatalytic properties. It was immobilized on a glassy carbon electrode (GCE) with chitosan (CHIT) to fabricate a new kind of electrochemical sensor, EPC/CHIT/GCE, which showed excellent electrocatalytic activity in the reduction of H2O2. Meanwhile, EPC could also promote electron transfer with the help of hydroquinone. The simple and low-cost electrochemical sensor exhibited high sensitivity, and good operational and long-term stability.
Highlights
The accurate and sensitive detection of H2 O2 content is of great significance in clinical diagnostics, food safety, environmental monitoring and other fields [1]
It is generally believed that ampere-based electrochemical biosensors based on fixed horseradish peroxidase (HRP) are the most effective and commonly used methods for detecting H2 O2
The denaturation and shedding of surface enzymes on the sensor electrode of immobilized enzymes and other bioprotein molecules has been a major obstacle to the application of enzyme sensors, especially commercial applications, because it directly affects the sensitivity, stability and repeatability of the sensor
Summary
The accurate and sensitive detection of H2 O2 content is of great significance in clinical diagnostics, food safety, environmental monitoring and other fields [1]. More detection techniques such as titration analysis [2], spectrophotometry [3], chemiluminescence [4], and electrochemical methods [5] are used to analyze H2 O2. Among these methods, electrochemical methods are widely used and are widely reported. The denaturation and shedding of surface enzymes on the sensor electrode of immobilized enzymes and other bioprotein molecules has been a major obstacle to the application of enzyme sensors, especially commercial applications, because it directly affects the sensitivity, stability and repeatability of the sensor. It is still a key issue in enzyme sensors that has to be overcome so far
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