Abstract
AbstractMicrobial consortia have great potential for the production of polyhydroxyalkanoates (PHAs) due to their comprehensive capability. In this study, a microbial consortium consisting of Pseudomonas putida NBRC14164 and Saccharomyces cerevisiae SyBE_Sc01020078 was constructed where xylose was converted into nutrients utilizable to P. putida by S. cerevisiae to realize the production of medium‐chain‐length polyhydroxyalkanoates (mcl‐PHAs) from xylose and octanoate, followed by a preliminary analysis of the interaction between the two strains. The optimal inoculation ratio consisted of 1% P. putida and 10% S. cerevisiae, and both strains exhibited the highest PHA production at their mid‐exponential phase. The two microorganisms were grown in a mineral salt medium with xylose and octanoate as substrates without washing and cultured at 200 rpm. The mcl‐PHA titre of this consortium was 295.7 mg/L, which was 150% higher than that of P. putida in pure culture. Metabolites analysis by gas chromatography–mass spectrometry revealed metabolic communication between P. putida and S. cerevisiae, whereby the former provided histidine, methionine, and leucine for the latter, while the latter converted xylose into lactic acid, which is a more accessible carbon source for the former.
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