Abstract
Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH2-Alginate, is synthesized through an oxidation-amination-reduction process. The “catalysis function” ensures the as-prepared NH2-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na2SiO3), and the “template function” cause microscopic phase separation in aqueous solution. The diameter of resultant NH2-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH2-Alginate. The size and morphology of obtained NH2-Alginate/silica nanocomposite are correlated with the micelles. NH2-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH2-Alginate and cage effect of silica matrix.
Highlights
Recent years, polymer-silica nanocomposite have aroused a lot of interest from the viewpoints of both scientific research and technological applications [1,2,3]
Poulsen [20] proved that long-chain polyamines (LCPA) could undergo microscopic phase separation to form a microemulsion template, which significantly influenced the microstructure of polymer-silica nanocomposite
A significant increase in nitrogen content demonstrates that the amine groups are successfully grafted
Summary
Polymer-silica nanocomposite have aroused a lot of interest from the viewpoints of both scientific research and technological applications [1,2,3]. Poulsen [20] proved that long-chain polyamines (LCPA) could undergo microscopic phase separation to form a microemulsion template, which significantly influenced the microstructure of polymer-silica nanocomposite. Other multivalent anions [21,22] such as pyrophosphate, sulfate, or polyanion natSil-2 [20] were capable of inducing the precipitation of silica from silicate-containing polyamine solutions. Kroger [20] reported that natSil-1A could form the microscopic phase separation as template and induce silica-precipitating as catalyst, even in the absence of polyanions or multivalent anions. It can be explained that all the serine residues are phosphorylated and all the lysine residues are either methylated or covalently linked with polyamines This structure ensures a highly zwitterionic polypeptide with the capability for self-assembly, which leads the microscopic phase separation driven by ionic interactions [23,24].
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