Abstract
Although nitrocellulose (NC) membrane as a substrate material has been extensively utilized to fabricate lateral flow assays (LFAs), it still faces many challenges in the sensitivity of the assay. Herein, a facile strategy for improving LFAs sensitivity was developed by incorporating two kinds of electrospun fibrous membranes (EFMs) into LFAs: one composes of microfibers (MFMs), and the other consists of nanofibers (NFMs). Kanamycin (Kna) was recruited as the model target to demonstrate the concept, and a systematic study in optimizing assay parameters was carried out. The investigation confirmed that EFMs could regulate the flow rate of samples and the biomolecules' immobilization capacity of the substrate. Under optimal conditions, NFMs-based LFAs perform better than NFMs, achieving a naked eye detection limit of 0.25 ng/mL and excellent selectivity. Compared with the NC-based LFA, the results showed a twenty-fold increase in the sensitivity for Kna. Moreover, further studies have verified the applicability of LFAs in actual samples. The proposed method can be easily extended to sensitive detection of other targets by replacing the corresponding biomolecules, paving a new avenue for food safety, biomedical, and environment monitoring.
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