Abstract
An amylolytic brewing yeast Saccharomyces pastorianus , free of vector sequences and drug-resistance genes, was constructed by disrupting the α-acetolactate synthase gene and introducing the α-amylase gene as a selective marker. The resulting recombinant strain was able to utilize starch as the sole carbon source and its α-acetolactate synthase activity was lowered by 30%. Fermentation tests confirmed that the diacetyl concentration and the residual oligosaccharide were reduced by 70% and 25%, respectively, in fermented wort by the recombinant strain, while the brewing performance of the recombinant strain was retained.
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