Abstract
A nuclear protooncogene c- myb has been hypothesized to play an important role in hematopoiesis, but little is known about the physiological function of the c- myb gene products. To study the role of c- myb gene expression in monocyte-macrophage differentiation and proliferation, we introduced exogenous c- myb gene into murine myelomonocytic leukemia WEHI-3B(D+) cells which can be induced to differentiate into mature monocytes with granulocyte-colony stimulation factor (G-CSF) and actinomycin D. Expression of the transfected gene was found to result in elevated levels of c- myb transcripts, which were not subject to normal down-regulation by differentiation induction. This constitutive expression of c- myb gene allowed the c- myb transfectants to differentiate into promonocytes with G-CSF and actinomycin D, but blocked further maturation from promonocytes to mature monocytes. It is concluded that normal down-regulation of c- myb gene expression during monocyte-macrophage differentiation is required for the maturation of promonocytes to mature monocytes.
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