Constituents from the Fruiting Bodies of Trametes cubensis and Trametes suaveolens in Vietnam and Their Anti-Inflammatory Bioactivity.

Yue-Chiun Li,Tran Dinh Thang,Tsong-Long Hwang,Tian-Shung Wu,Ping-Chung Kuo,Nguyen Thi Ngan,Nguyen Ngoc Tuan,Mei-Lin Yang,Kun-Ching Cheng

doi:10.3390/molecules26237311

Abstract

It is reported that various fungi have been used for medicine and edible foods. The tropical Trametes genus is popular and well-known in Vietnam for its health effects and bioactivities. In this study, the fruiting bodies of the edible fungi T. cubensis and T. suaveolens were collected in Vietnam. The preliminary bioactivity screening data indicated that the methanol extracts of the fruiting bodies of T. cubensis and T. suaveolens displayed significant inhibition of superoxide anion generation and elastase release in human neutrophils. Therefore, the isolation and characterization were performed on these two species by a combination of chromatographic methods and spectrometric analysis. In total, twenty-four compounds were identified, and among these (1–3) were characterized by 1D-, 2D-NMR, and HRMS analytical data. In addition, the anti-inflammatory potentials of some purified compounds were examined by the cellular model for the inhibition of superoxide anion generation and elastase release in human neutrophils. Among the isolated compounds, (5,14), and (19) displayed significant anti-inflammatory potential. As the results suggest, the extracts and isolated compounds from T. cubensis and T. suaveolens are potential candidates for the further development of new anti-inflammatory lead drugs or natural healthy foods.

Highlights

IntroductionOnce the human body is stimulated by bacteria, viruses, wounds, or other environment factors, the immune system will respond by inflammation to resist the infection and irritation
The fruiting bodies of T. cubensis and T. suaveolens were extracted with methanol, and partitioned with dichloromethane and water to give aliphatic and water soluble layers
(Figure 2) were characterized and their chemical structures were constructed with the assistance of the nuclear magnetic resonance (NMR) spectral elucidation and MS spectrometric analysis

Summary

Introduction

Once the human body is stimulated by bacteria, viruses, wounds, or other environment factors, the immune system will respond by inflammation to resist the infection and irritation. When organs and tissues are in inflammation, neutrophils are usually the first lymphocytes to reach the infected region [1]. Various cytotoxins, such as superoxide anion and elastase can be secreted by neutrophils in response to activation of the immune system [2]. Coussens et al showed the relationship between inflammation and cancer, and they indicated that the formation of cancer cells is attributed

Methods

Results

Conclusion