Abstract
The hydro-distillation of aerial parts of Artemisia lehmanniana was analyzed by a combination of GC and GC-MS. Twenty-seven compounds, accounting for 92.1 % of the total oil, were identified. The main components of the essential oil were camphor (31.5 %), 1,8-cineole (16.9 %) and camphene (8.0 %). The oil was found to be rich in regard to oxygenated monoterpenes (65.1 %). The antimicrobial activity of essential oil was determined against eight bacteria and two fungal strains. The bacteria included Bacillus cereus, Bacillus subtilis, Enterobacter spp, Escherichia coli, Citrobacter spp, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus and Candida albicans and Aspergillus niger were used as fungi. The oil has shown maximum zone of inhibition against Enterobacter spp, Staphylococcus aureus, Candida albicans and Aspergillus niger (10 mm). Bacillus subtilis and Aspergillus niger were the most (having MIC value 2.5 mg/ml and MBC and MFC value 2.5 mg/ml respectively) and Escherichia coli and Pseudomonas aeruginosa were the least sensitive microorganisms to the essential oil (MIC value 10.0 mg/ml and MBC 10.0 mg/ml respectively). The essential oil had a moderate antimicrobial activity.
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