Consistent increase in abundance and diversity but variable change in community composition of bacteria in topsoil of rice paddy under short term biochar treatment across three sites from South China

Abstract

Biochar functionality related to soil microbial community changes has not yet been fully understood. In this study, we present a cross site field experiment on bacterial community changes of rice paddies among three sites (Jiangxi province, JX; Hunan province, HN; and Sichuan province, SC) from South China with biochar amended (BSA) at 0, 20 and 40t ha−1 before rice plantation in 2010. Changes in bacterial abundance and diversity of topsoil (0-15cm) sampled at rice harvest were assessed. Increases in soil pH, soil organic carbon, total N, soil microbial biomass, as well as bacterial gene copy numbers and diversity indices (phylogenetic diversity, Shannon, Chao1 and OTU richness) were consistently observed under BSA at 40t ha−1, though generally insignificant at 20tha−1 across the sites. Cluster analysis of both terminal restriction fragment length polymorphism (T-RFLP) profiles and pyrosequencing of the 16S gene indicated a strong impact of biochar on bacterial community composition, though the changes were variable across the sites. In particular, BSA at 20 and 40tha−1 greatly increased the relative abundance of Betaproteobacteria (by 54% and 80%) and Deltaproteobacteria (by 164% and 151%) in JX while decreased Betaproteobacteria (by 46% and 52%) and increased Chloroflexi (by 27% and 61%) in SC site, respectively. However, no significant changes were detected in HN site. In addition, some significant but variable changes were observed in the abundance of nitrifying, denitrifying and N-fixing bacteria groups with biochar addition among sites. This study suggested a potential role of biochar in enhancing bacterial abundance, community diversity and modifying the community compositions, particularly of the bacteria involved in N cycling. However, changes in soil microbial structure and functioning related to biochar treatment deserve further studies.