Conservation of vitamin C by uric acid in blood

Abstract

Urate at physiological concentrations increased the stability of ascorbate in human serum approximately fivefold. These measurements were made by depleting human serum of urate with uricase. In model experiments (using phosphate buffer instead of serum), urate protected against iron-induced ascorbate oxidation and, to a lesser extent, pH-induced ascorbate oxidation. In both human serum and in phosphate buffer, urate exerted its protective effect without itself undergoing measurable oxidation as determined by spectrophotometric and high pressure liquid chromatography (HPLC) techniques. These experiments suggest an important physiological role for urate in preserving ascorbate in blood and other biological fluids.