Conservation of Properties of Outer Membranes Protein across Host Genera of Pasteurella multocida Suggests Common Mechanism of Action

Monendra Grover

doi:10.4172/2168-9547.1000161

Abstract

Pasteurella multocida is a non-motile coccobacillus pathogenic Gram-negative bacterium and belongs to Pasteurellaceae family. Pasteurella multocida causes diseases in economically important animals and birds in developing and developed countries. Haemorrhagic septicaemia in cattle and buffaloes and other diseases like fowl cholera (turkey, chicken, and duck), Septicaemic pasteurellosis (sheep, pig, and goat) and Snuffles (rabbit) are caused by Pasteurella multocida. In this analysis we have taken three outer membrane proteins (vacJ, ompW and skp) from P. multocida which are involved in infectious diseases of animals and birds. The literature shows that all three outer membrane proteins are infectious in nature. This is supported by multiple sequence alignment and analysis of physicochemical properties of protein sequences encoded by vacJ, ompW and skp outer membrane protein families. The studied proteins are similar with respect to number of positively and negatively charged amino acids, molecular weight, Theoretical pI, instability index and grand average of hydropathicity (Gravy) in the proteins from P. multocida strains infecting different genera. The domains, transmembrane helices, twin arginine signal peptides and s-barrels are also broadly similar. This suggests common mechanism of action of these proteins across host genera.

Highlights

Pasteurella multocida has been classified on the basis of properties and reactions of blood serum and disease that are designated by five capsular serogroups A, B, C, D, E, F, and 16 types of serotypes [1]
The local alignment was performed using BLAST against non-redundant protein sequences database for searching the conserved domains for all 23 sequences that belong to vacJ, skp and Outer Membrane Protein W (ompW) outer membrane proteins
The analysis showed that vacJ and ompW proteins in domesticated ruminant mammal sheep and goat are interestingly different in comparison to the proteins from strains of P. multocida infecting other animals, whereas, skp outer membrane protein is found to be similar in all the cases

Summary

Introduction

Pasteurella multocida has been classified on the basis of properties and reactions of blood serum and disease that are designated by five capsular serogroups A, B, C, D, E, F, and 16 types of serotypes [1]. The main hosts of haemorrhagic septicaemia infection and disease are buffalo and cattle but other animals like goats, pigs, deer, sheep and camel are susceptible to it. The respiratory tracts of warm-blooded animals may be affected by diseases like haemorrhagic septicaemia, fowl cholera and snuffles [2]. Pasteurella multocida serotype B: 2 is the etiological agent of haemorrhagic septicaemia (HS) in cattle and buffaloes, a disease that causes significant economic losses to the dairy industry in India [3]. Haemorrhagic septicaemia is caused by two particular serotypes of Pasteurella multocida: B: 2 and E: 2. It is known to be associated with the localisation of bacteria in the tonsils of living buffalo, showing that animals can become transporters [4]

Methods

Discussion

Conclusion