Abstract
This research examined the influence of the addition of L-carnitine (LC) to cooling medium on buck's semen quality during cooling storage periods at 4oC. Semen samples were collected, diluted, assigned into four groups, and received LC (0, 1, 5, and 10 mM LC). The samples were then chilled to 4oC and stores for 48 h. Sperm total motility, progressive motility, viability, lipid peroxidation, membrane integrity, and mitochondrial activity were examined at 0, 24, and 48 h of cooling storage. At time 0 of cooling storage, different treatments showed no impact on the quality of sperm samples (P>0.05). During 24 and 48 h of chilling periods, the supplementation of cooling medium with 5 mM LC presented greater motility, viability, membrane integrity, and mitochondrial activity (P≤0.05), compared to the other groups. Moreover, the treatment of 5 mM LC caused lower lipid peroxidation (P≤0.05) than the other treatments at 24 and 48 h storage times. In conclusion, the supplementation of buck's cooling storage medium with 5 mM LC is a suitable way to protect buck spermatozoa during 24 and 48 h storage against cold-induced structural and functional damages.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
