Consequences of sodium dodecyl sulfate exposure on the antioxidant status and steroidogenesis in fish gonad

Abstract

A native Indian catfish (Heteropneustes fossilis) and an Indian major carp (Cirrhinus cirrhosus) were treated with sodium dodecyl sulfate (SDS) to elucidate its consequences on antioxidant status and sex steroid synthesis. Fishes were exposed to 1/10th of LC50 of SDS for 30 days. Different enzymatic and non-enzymatic antioxidants, major sex steroids, steroidogenic enzymes, gonadosomatic index (GSI), and serum cortisol level were measured in both male and female fishes. Levels of enzymatic and non-enzymatic antioxidants altered significantly in both the fish species. Steroid hormones in both male and female fishes decreased significantly in the SDS-treated group. Profiles of steroidogenic enzymes (hydroxysteroid dehydrogenase; 3β-HSD and 17β-HSD) show significant decrease in the SDS treatment group in both carp and catfish than that of the control group irrespective of sexes. GSI also decreases significantly in the SDS-treated group when compared with the control group in spawning phase of carp and catfish irrespective of sexes. Serum cortisol level was increased significantly after SDS exposure. Higher level of cortisol was noted in the spawning phase than that of pre-spawning phase. Current study proves that SDS could interrupt the normal steroidogenesis process in fish, leading to impaired sex hormone production. Detrimental effect of SDS was noted to be more prominent on female steroidogenesis than that of male fish. A species or season-specific variation in steroidogenesis was also observed which indicates that SDS-induced toxicity depends on the sexual status of the specific fish species.