Abstract
BackgroundFlax is an important field crop that can be used for either oilseed or fiber production. Plant height and technical length are important characters for flax. For linseed flax, plants usually have a short technical length and plant height than those for fiber flax. As an important agronomical character for fiber and linseed flax, plant height is usually a selection target for breeding. However, because of limited technologies and methods available, there has been little research focused on discovering the molecular mechanism controlling plant height.ResultsIn this study, two related recombinant inbred line (RIL) populations developed from crosses of linseed and fiber parents were developed and phenotyped for plant height and technical length in four environments. A consensus linkage map based on two RIL populations was constructed using SNP markers generated by genotyping by sequencing (GBS) technology. A total of 4497 single nucleotide polymorphism (SNP) markers were included on 15 linkage groups with an average marker density of one marker every 2.71 cM. Quantitative trait locus (QTL) mapping analysis was performed for plant height and technical length using the two populations. A total of 19 QTLs were identified for plant height and technical length. For the MH population, eight plant height QTLs and seven technical length QTLs were identified, five of which were common QTLs for both traits. For the PH population, six plant height and three technical length QTLs were identified. By comparing the QTLs and candidate gene information in the two population, two common QTLs and three candidate genes were discovered.ConclusionsThis study provides a foundation for map-based cloning of QTLs and marker-assisted selection for plant height-related traits in linseed and fiber flax.
Highlights
Flax is an important field crop that can be used for either oilseed or fiber production
The correlation analysis showed that the two traits in the two populations were ddRADseq statistics for the two recombinant inbred line (RIL) populations The digested restriction site-associated DNA (ddRAD) seq protocol was used to construct sequence libraries for both the Macbeth/Heiya No.14 (MH) and plant height (PH) RIL populations
Differing from previously published linkage maps in flax, the molecular markers used in this study were single nucleotide polymorphism (SNP) markers based on the high-throughput sequencing technology-ddRAD sequencing method
Summary
Flax is an important field crop that can be used for either oilseed or fiber production. Plant height and technical length are important characters for flax. As one of the important agronomical characters for both fiber and linseed flax, plant height is usually a selection target in breeding. Contreras-Soto et al found that some SNP markers on Chr controlled both the plant height and seed weight traits through a genome-wide association study (GWAS) in soybean [5]. Technical length is another selective breeding target in flax. There has been no research on plant height and technical length gene discovery in flax to date
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