Connective tissue components in blood, urine, and bone following a massive dose of parathyroid extract

Abstract

1. 1. A single dose of parathyroid extract (PTE) produced a prompt increase in the urinary excretion of hydroxyproline and glucosamine. The hydroxyproline specific activity and the glucosamine specific activity were also increased. 2. 2. The hydroxyproline and hydroxyproline specific activity increased in the serum within 12 hr following PTE administration and returned to baseline levels within 24 hr. Serum calcium and glucosamine and serum glucosamine radioactivity determinations remained elevated at both time periods following PTE administration. 3. 3. The amount of collagen extracted from metaphyseal bone after PTE was below the amount extracted from controls, whereas the glucosamine content of bone remained unchanged after PTE. These changes were reflected in the radioactivity of combusted bone samples. Combusted bone from the treated animals contained an increased amount of 14C 24 hr after PTE.