Abstract
It has been hypothesized that the cytotoxicity of secondary organic aerosols (SOA) is mediated through the formation of reactive oxygen species (ROS) in the exposed cells. Here, lung epithelial cells (A549) residing at the air-liquid interface were exposed to proxies of anthropogenic and biogenic SOA that were photochemically aged under varying nitrogen oxide (NOx) concentrations in an oxidation flow reactor. The total organic peroxides and ROS radical content in the SOA were quantified by the iodometric spectrophotometric method and by continuous-wave electron paramagnetic resonance. The effect of the exposure was evaluated by measuring cell viability and cellular ROS production following the exposure. The results demonstrate that SOA that aged in the absence of NOx contained more ROS than fresh SOA and were more toxic toward the cells, while varying NOx conditions had no significant influence on levels of the ROS content in fresh SOA and their toxicity. Analysis of ROS in the exposed cells using flow cytometry showed a similar trend with the total ROS content in the SOA. This study provides a first and direct observation of such association.
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