Abstract
Interspecies transfer of the conjugative transposons Tn916 and Tn925 into B. popilliae Pj1 occurred using Enterococcus faecalis and Bacillus subtilis CU4049 as transposon donors. Tn916 was stably maintained in B. popilliae Pj1 following growth without selective pressure and was successfully introduced into the plasmid-containing B. popilliae strains NRRL B-2524, Ch1, and KLN4 using E. faecalis CG110. In B. popilliae, expression of the tetracycline resistant determinants on Tn916 and Tn925 provided resistance to 25 μg/mL and 50 μg/mL tetracycline, respectively. An erythromycin resistant determinant, present in Tn916ΔE, was also functional in B. popilliae Pj1 and provided resistance to 1 mg/mL erythromycin. Transfer of Tn916 into E. faecalis, B. subtilis, and between B. popilliae strains was accomplished using a transposon-containing strain of B. popilliae as donor. Efforts to transfer Tn916 between E. coli and B. popilliae were unsuccessful. Key words: Bacillus popilliae, milky disease, Tn916, conjugative transposon.
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