Abstract
RA1 was the only IncC plasmid that was slightly temperature-sensitive for replication and transfer. At 30°C, RA1 determined constitutive synthesis of conjugative pili and yet was transfer-repressed. Attachment of shaft-adsorbing RNA phage C-1 virions prevented the probable retraction of pili under heat stimulus (55°C). Electron microscopy showed single adsorbed virions at pilus bases where they were thought to have stopped retraction.
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