Abstract
New materials that are as similar as possible in terms of structure and biology to the extracellular matrix (external environment) of cells are of great interest for regenerative medicine. Oligoproline and oligohydroxyproline derivatives (peptides 2–5) are potential mimetics of collagen fragments. Peptides 2–5 have been shown to be similar to the model collagen fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH, 1) in terms of both their spatial structure and biological activity. In this study, peptides 2–5 were covalently bound to nonwovens based on chitosan and calcium alginate. Incorporation of the peptides was confirmed by Fourier transform -infrared (FT-IR) and zeta potential measurements. Biological studies (cell metabolic activity by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and Live/Dead assay) proved that the obtained peptide-polysaccharide conjugates were not toxic to the endothelial cell line EA.hy 926. In many cases, the conjugates had a highly affirmative influence on cell proliferation. The results of this study show that conjugates of chitosan and calcium alginate with oligoproline and oligohydroxyproline derivatives have potential for use in regenerative medicine.
Highlights
The aim of biomimetics of the cell environment is to constitute a matrix for cell deposition that affects cells in such a way that they are able to function as if they were in their natural environment [1].materials used as cell scaffolds must provide an appropriate cell microenvironment for adequate tissue growth [2,3]
We investigate whether oligoproline and oligohydroxyproline derivatives can be used as conformationally stable collagen fragment mimetics in regenerative medicine
We examine whether the covalent attachment of oligoproline derivatives to polysaccharides allows for the improvement of conjugate activity compared to the individual components
Summary
The aim of biomimetics of the cell environment is to constitute a matrix for cell deposition that affects cells in such a way that they are able to function as if they were in their natural environment [1].materials used as cell scaffolds must provide an appropriate cell microenvironment for adequate tissue growth [2,3]. Synthetic scaffolds characterized by biodegradability and the possibility of modulating their mechanical and physical properties have been developed, with structural parameters adapted to the needs of various types of tissues The problem with this group of compounds is that they can cause acidification of the environment, resulting from the degradation of polyesters to the corresponding mers. Hybrid scaffolds, resulting from the merger of synthetic and natural materials, have proven be useful in regenerative medicine Their formation involves the modification of surface of synthetic polymers using biomolecules found in extracellular matrix (ECM) such as fibrinogen or collagen. This improves their properties and biocompatibility, combining the advantages of both types of structure—the mechanical strength of synthetic polymers and the ability of natural compounds to interact with cells [11,12]
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