Conjugated linoleic acid promotes endurance capacity via SIRT1 and AMPK mediated mechanisms (37.3)

Abstract

Previously it was reported that conjugated linoleic acid (CLA) and exercise training improves endurance capacity potentially via peroxisome proliferator‐activated receptor δ (PPARδ)‐mediated mechanism in mice. Based on these results, the current study was designed to determine the role of exercise and/or CLA in improved endurance capacity and upstream regulator(s) for PPARδ. Male 129Sv/J mice were fed either control or CLA (0.5%) containing diet for 4 weeks and further divided into sedentary or training regime (25 min three times per week). Consistent with previous observations, CLA feeding significantly reduced body weight and fat mass independent of excise during the experimental period. Endurance capacity was significantly improved by CLA treatment, while there was no significant effect of training alone. Similarly, CLA treatment significantly increased expression of peroxisome proliferator‐activated receptor gamma coactivator 1‐α (PGC1α), an upstream regulator of PPARδ, in both sedentary and trained animals, 58% and 28%, respectively. It was further determined that CLA influences silent information regulation 2 homolog‐1 (SIRT1) and AMP‐activated protein kinase (AMPK) regardless of activity levels, which are upstream regulators of PGC1α. These results suggest that CLA improves exercise outcome independent of activity levels via SIRT1 and AMPK mediated mechanisms.