Abstract
Metabolic diseases such as obesity and diabetes are increasing rapidly, leading to adverse effects on patients’ intestinal function and health. In this study, high-purity conjugated linoleic acid (CLA) was extracted using glycerol from Suaeda salsa, followed by purification using simulated moving bed chromatography technique. Subsequently, type 2 diabetic mellitus (T2DM) mice were administered c9,t11-CLA (100 mg/kg), t10,c12-CLA (100 mg/kg), or varying doses of high-purity CLA diet (100, 600, or 1800 mg/kg) for a duration of five weeks. The impact on intestinal function was evaluated through morphological and tissue function studies combined with 16S rDNA sequencing technology and other techniques. The results demonstrated that the CLA was purified to 97.81% and significantly reduced blood glucose and lipid levels in T2DM mice by inhibiting the expression of small intestinal glucose and lipid transporters; enhanced colon barrier function by upregulating the expression of colon tight junction proteins; activated Jnk-c-Jun pathway to suppress intestinal cell apoptosis thereby improving survival rate; improved intestinal microecological health by modulating intestinal flora composition and metabolic function. These findings demonstrate the potential advantageous impact and mechanism of CLA derived from S. salsa in enhancing intestinal health among individuals with T2DM, providing a substantial theoretical and practical foundation for investigating the hypoglycemic effects of CLA and addressing global public health concerns.
Published Version
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