Abstract

At present, there is limited and contradictory information about the effects of the use of canola (Brassica napus) seed as supplement on the contents of conjugated linoleic acid (CLA) in milk of grazing cows. The objective of this study was to evaluate the effect of a dietary supplement with canola seed on the production and composition of milk, and CLA concentration in Chilean Black Friesian cows under pasture conditions. Three experiments were done. Experiment 1: control group was fed 5 kg d-1 of commercial concentrate without canola (0-TC1) and treatment group that was fed 3.75 kg of commercial concentrate plus 1.16 kg of whole canola seed (1.16-TC1). Experiment 2: Control group was fed 8 kg d-1 commercial concentrate without canola (0-TC2) and treatment group that was fed 6.2 kg of commercial concentrate plus 1.2 kg of ground canola seed (1.2-TC2). Experiment 3: control group was fed 6 kg d-1 commercial concentrate without canola (0-TC3) and treatment group was fed 6 kg of commercial concentrate with 20% of whole canola seed (1.2 kg d-1, 1.2-TC3). The duration of each experiment was 60 days. No differences in milk production and quality were observed among the experimental groups in every assay. The CLA isomers trans-10, cis-12 and cis-10, cis-12 were higher than those normally found in the scientific literature. There was no effect of the inclusion of canola seed on total CLA content or the content of cis-9, trans-11, trans-10, cis-12 and cis-10, cis-12 isomers.

Highlights

  • Conjugated linoleic acid (CLA) has been indicated as one of the most beneficial fatty acids for human health (Pariza & Park, 2001)

  • There was no effect of the inclusion of canola seed on total conjugated linoleic acid (CLA) content or the content of cis-9, trans-11, trans-10, cis-12 and cis-10, cis-12 isomers

  • Diet has a major influence on milk fat CLA content and it has been extensively investigated (Bauman et al, 2001)

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Summary

Introduction

Conjugated linoleic acid (CLA) has been indicated as one of the most beneficial fatty acids for human health (Pariza & Park, 2001). Studies in animal models and human cell lines have provided evidence that certain isomers of CLA, including cis-9, trans-11 CLA, exhibit potent antiinflammatory, immunomodulatory, antiobesity, and anticarcinogenic activities; and improve biomarkers of cardiovascular health (Wahle et al, 2004; Shingfield et al, 2008). The content of CLA in fat from ruminant-derived foods is dependent on the ruminal production of both CLA, trans-vaccenic acid (TVA), and on the tissue activity of Δ9-desaturase. It has been reported that fresh forage (Khanal et al, 2005; Dewhurst et al, 2006; Butler et al, 2008; Abbreviations used: ADF (acid detergent fibre); BCS (body condition score); CLA (conjugated linoleic acid); DIM (days in milk); DM (dry matter); FA (fatty acid); FCM (fat corrected milk production); GLM (general linear model); ME (metabolizable energy); NDF (neutral detergent fibre); TC (treatment canola); TMR (total mixed ration); TVA (trans-vaccenic acid)

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