Abstract
Summary A Tn 916 -like transposon (Tn FO1 ) was found in the multiple antibiotic resistant Enterococcus faecalis strain FO1 isolated from a raw milk cheese. In this strain, the tetracycline determinant was localized by DNA-DNA hybridization with a tetM nucleotide probe on the chromosome and on a 30-kb plasmid. The transposon Tn FO1 was identified and characterized by DNA-DNA hybridization experiments with the five internal Hinc II fragments of Tn 916 . The tetracycline resistance determinant was identified by its complete nucleotide sequence as TetM. Transposon Tn FO1 was also detected in its circular form by DNA-DNA hybridization and PCR amplification. Both ends including the joining region of the closed circular transposon Tn FO1 were sequenced. Tn FO1 could be transferred by conjugation from Enterococcus faecalis into Enterococcus faecalis, Lactococcus lactis subsp. lactis biovar. diacetylactis, Listeria innocua, Leuconostoc mesenteroides and Staphylococcus aureus , and from Lactococcus lactis subsp. lactis biovar. diacetylactis into Listeria innocua . Pulsed-field electrophoresis of genomic DNA from E. faecalis FO1 transconjugants showed that transposon Tn FO1 integrated at different sites.
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