Abstract
In the present study, we have investigated some growth conditions capable of inducing the conidial germination in Scedosporium apiospermum, S. aurantiacum, S. minutisporum and Lomentospora prolificans. Germination in Sabouraud medium (pH 7.0, 37ºC, 5% CO2) showed to be a typically time-dependent event, reaching ~75% in S. minutisporum and > 90% in S. apiospermum, S. aurantiacum and L. prolificans after 4 h. Similar germination rate was observed when conidia were incubated under different media and pHs. Contrarily, temperature and CO2 tension modulated the germination. The isotropic conidial growth (swelling) and germ tube-like projection were evidenced by microscopy and cytometry. Morphometric parameters augmented in a time-dependent fashion, evidencing changes in size and granularity of fungal cells compared with dormant 0 h conidia. In parallel, a clear increase in the mitochondrial activity was measured during the transformation of conidia-into-germinated conidia. Susceptibility profiles to itraconazole, fluconazole, voriconazole, amphotericin B and caspofungin varied regarding each morphotype and each fungal species. Overall, the minimal inhibitory concentrations for hyphae were higher than conidia and germinated conidia, except for caspofungin. Collectively, our study add new data about the conidia-into-hyphae transformation in Scedosporium and Lomentospora species, which is a relevant biological process of these molds directly connected to their antifungal resistance and pathogenicity mechanisms.
Highlights
In the present study, we have investigated some growth conditions capable of inducing the conidial germination in Scedosporium apiospermum, S. aurantiacum, S. minutisporum and Lomentospora prolificans
Biochemical and genetic features, some species of Pseudallescheria and Scedosporium were allocated in a fungal complex designated as Pseudallescheria/Scedosporium complex, which is currently composed by Pseudallescheria boydii, Scedosporium apiospermum, S. dehoogii, S. aurantiacum and S. minutisporum (Gilgado et al 2010, Lackner et al 2014)
In order to add new data on this relevant subject, in the present study we have investigated some physicochemical conditions able to induce the conidial germination in S. apiospermum, S. aurantiacum, S. minutisporum and L. prolificans, including culture medium composition, pH, temperature and CO2 tension
Summary
We have investigated some growth conditions capable of inducing the conidial germination in Scedosporium apiospermum, S. aurantiacum, S. minutisporum and Lomentospora prolificans. We initially analysed the time-dependence kinetics of the morphological transformation after incubation of S. apiospermum, S. aurantiacum, S. minutisporum and L. prolificans conidial cells in Sabouraud medium (pH 7.0) at 37oC up to 4 h in an atmosphere of 5% CO2 (Fig. 2).
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