Abstract
The discrimination of species in the genus Anuraphis is particularly difficult due to the overlap of morphological characters. In this study, we used the 5’ (barcode) and 3’ regions of cytochrome oxidase I (COI) to test their utility in the identification of species in this genus as well as closely related species. Both regions were useful to discriminate all the species tested. However the non-barcode 3’ region resulted in higher resolution and support for species relationships when the data were analyzed using both Maximum Likelihood and MrBayes. We propose the development of an integrated database that encompasses morphological, molecular, life-cycle, host plant and bibliographic information to facilitate and increase the accuracy of aphid identification.
Highlights
The genus Anuraphis Del Guercio presently ascribed to the tribe Macrosiphini includes a small number of taxonomically welldefined species, A. subterranea (Walker, 1852), A. farfarae (Koch, 1854), A. catonii Hille Ris Lambers, 1935, A. pyrilaseri Shaposhnikov, 1950, A. cachryos Barbagallo & Stroyan, 1982, A. ferulae Shaposhnikov, 1995 and A. shaposhnikovi Barbagallo & Cocuzza, 2003
The molecular analysis based on the 3’ and 5’ cytochrome oxidase I (COI) gene regions indicates that the genus Anuraphis is a homogeneous taxonomic group
The division of Anuraphis species in two groups is observable by comparing the phylogenetic trees and Mahalanobis’ generalized distance
Summary
The genus Anuraphis Del Guercio presently ascribed to the tribe Macrosiphini includes a small number of taxonomically welldefined species, A. subterranea (Walker, 1852), A. farfarae (Koch, 1854), A. catonii Hille Ris Lambers, 1935, A. pyrilaseri Shaposhnikov, 1950, A. cachryos Barbagallo & Stroyan, 1982, A. ferulae Shaposhnikov, 1995 and A. shaposhnikovi Barbagallo & Cocuzza, 2003. The generic placement of A. capparidis has been questioned by Blackman and Eastop (2006) who noted that, based on the original description, this is probably not an Anuraphis species but an immature Aphis sp. A common trait of almost all Anuraphis species is the use of Apiaceae as host plants, with the exception of A. farfarae that feeds on Asteraceae (Tussilago, Petasites and Hieracium). Some populations of A. farfarae (Shaposhnikov & Sharov, 1978), and probably other species, are solely anholocyclic on secondary host plants. For A. cachryos, A. shaposhnikovi and A. ferulae the primary host plants remain to be determined
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
