Congenital Duodenal Atresia in Rat Model Induced by Adriamycin

Abstract

Objective To describe a reproducible fetal model of duodenal atresia.Methods Timemated pregnant rats were given 1.75 mg/kg of Adriamycin intraperitoneally on days 6 through 9 of gestation,and the litters were recovered on day 20.The fetuses were dissected microscopically and studied histologically.The findings were compared with those of age-matched saline embryos.Results All saline embryos were normal,whereas 81.7%(26/32)of Adriamycin embryos had duodenal atresia.The intraluminal atresia was found in 15.4%(4/26)of duodenal atresia embryos,gapped atresia with pancreatic tissue filled in between in 46.2%(12/26)and gapped atresia in 38.5%(10/26).A double duodenal atresia was found in one fetus.The malformation was anatomically identical to that of human neonates.Interestingly,all duodenal atresia embryos were associated with pancreatic agenesis,in 34.6% with the pancreatic neck,body and tail absence and in 65.4% with the body and tail absence.Conclusion This easily reproducible experimental model permits new research into both its embryogenesis and the biology of the duodenal atresia fetus. Key words: Adriamycin; Duodenal atresia