Abstract
By means of atomic force microscopy (AFM), the conformational structures of the circular double-stranded Deoxyribonucleic Acid (DNA) (2.96 kilobase pairs) on step-controlled silicon surface are studied. The DNA molecules observed on the terrace region maintain the supercoiled structure. On the other hand, the DNA molecules observed on the step-bunched region have a modified higher-order structure. Thus, we clarify that the conformations of long DNA are affected by the surface morphology of the substrate.
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