Abstract
The rice hoja blanca virus (RHBV), transmitted by the planthopper insect Tagosodes orizicolus, is a disease that attacks rice and generates significant production losses in Colombia. Fedearroz 2000 and Colombia I commercial rice varieties, which have different resistance levels to the disease, were selected in this study. To identify proteins associated to the insect and virus signaling, a comparative proteomics study was performed. By comparing proteomic profiles, between virus-infected and control group plants in two-dimensional electrophoresis, proteins exhibiting significant changes in abundance were found. In another test, peptide dendrimers containing sequences conformationally restricted to α-helix from four of those rice proteins were synthesized. In the experiment, sera from mice inoculated with peptide dendrimers could recognize the corresponding native protein in ELISA assays. Reported comparative proteomic results provide new insights into the molecular mechanisms of plant response to the RHBV and comprehensive tools for the analysis of new crop varieties. Besides, results from conformational peptide dendrimer approach are promising and show that it is feasible to detect proteins as markers, and may have biological applications by decreasing the susceptibility to proteolytic degradation.
Highlights
Rice (Oryza sativa L.) has become the most important food grain in Latin America and the Caribbean
The Fedearroz evaluation of the and results was made on the analysis of the three treatments: group; (2) vector without rice hoja blanca virus (RHBV); and (3) vector infected with RHBV, and compared for the two rice
RHBV produced chlorotic spots as well as streaks in the oldest leaves 30 days after inoculation. These results indicate that an alteration in the chloroplast metabolism is produced in response to RHBV
Summary
Rice (Oryza sativa L.) has become the most important food grain in Latin America and the Caribbean. Rice is considered a model species in molecular genetics because of the relatively small size of its genome. The rice genome consists of about 466 million base pairs and about 46,000 to. Molecules 2018, 23, 2262 genome has revealed extensive regions of preserved and ordered gene content, whose data can be used to perform various studies for improving this crop [1]. Rice hoja blanca (RHB) disease has been reported in tropical and subtropical American countries that grow rice. Unlike the phytosanitary situation of rice in Asia, RHBV was the only viral disease in America until 1991 when the rice stripe necrosis virus emerged in South America [6]. RHBV is transmitted by the planthopper insect Tagosodes orizicolus, which grows up to an average size of 2.5 mm
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