Abstract
Electrochemically induced three conformational transitions of calf thymus DNA from B 10.4 to Z 10.2-DNA and from B 10.2 to B 10.4 and to C-DNA in 10 mM phosphate buffer solution (pH 7.21) at glassy carbon electrode are found and studied by in situ circular dichroism (CD) thin layer spectroelectrochemistry with singular value decomposition least square (SVDLS) analysis. It indicates that the so-called B 10.2 form and the C-form of DNA may be composed of B 10.4 and left-A DNA and of B 10.4 and right-A DNA, respectively. The irreversible electrochemical reduction of adenine and cytosine groups in the DNA molecule is studied by UV-Vis spectroelectrochemistry. Some electrochemical parameters α n=0.17, E 0′=−0.70 V (vs. Ag/AgCl), and the standard heterogeneous electron transfer rate constant, k 0=1.8×10 −5 cm s −1, are obtained by double logarithmic analysis and non-linear regression.
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