Abstract
Fluorescence correlation spectroscopy was used to characterise the conformational equilibrium in “Calcium Green 1’', a fluorescent indicator of calcium. In the calcium bound form, a triplet bunching time of 6.7 µs is seen. We show that, in the absence of calcium, 10% of the indicator is in a fluorescent conformation that inter-converts to a non-fluorescent form in 10 µs. This confirms the probing mechanism based on a conformation controlled PET proposed for Calcium Green 1.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
