Abstract
We demonstrate that distance distributions between two sites on flexible molecules can be recovered using steady state measurements of fluorescence energy transfer. The characteristic Forster distance (R°) for energy transfer was varied by collisional quenching of the donor, which decreases its quantum yield. The measured transfer efficiencies for each value of R. provide a different average of the distance distribution. The R.-dependent transfer efficiencies, when analyzed by non linear least- squares, were found to reliably determine the distance distribution, as shown by agreement with the frequency-domain method (Chem. Phys. Lett. 1987, 138:587-593).© (1988) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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