Abstract
Knowledge of the sequence of different conformational states of a protein molecule is key to better understanding its biological function. A diffraction pattern from a single conformational state can be captured with an ultrafast X-ray Free-Electron Laser (XFEL) before the target is completely annihilated by the radiation. In this paper, we report the first experimental demonstration of conformation sequence recovery using diffraction patterns from randomly ordered conformations of a non-periodic object using the dimensional reduction technique Isomap and coherent diffraction imaging.
Highlights
A diffraction pattern from a single conformational state can be captured with an ultrafast X-ray Free-Electron Laser (XFEL) before the target is completely annihilated by the radiation
A diffraction pattern from a non-periodic object such as a large macromolecule or a virus may be captured by an ultrafast X-ray Free-Electron Laser (XFEL) before the target is completely annihilated by the radiation
We report the experimental demonstration of conformation sequence recovery using diffraction patterns from random instances of single micro-fabricated objects illuminated by soft X-ray FEL pulses
Summary
A diffraction pattern from a non-periodic object such as a large macromolecule or a virus may be captured by an ultrafast X-ray Free-Electron Laser (XFEL) before the target is completely annihilated by the radiation. This so called “Diffraction-before-destruction” technique [1,2] requires coherent X-ray pulses with higher peak brilliance than modern synchrotron sources can produce. The emergence of hard XFELs has enabled structural studies of nano-structured single particles [5,6,7] and microcrystals [8,9]. A pump-probe experiment at LCLS showed it may be possible to probe the conformational states of the structure of Photosystem II microcrystals [10] at particular times after exposure to an optical pulse
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
