Abstract
Background Designed Ankyrin Repeat proteins (DARPins) are a novel type of binding protein scaffold designed as antibody alternative for biomedical applications. DARPins share many properties with antibodies, most noteworthy a high target specificity and affinity, but they differ from antibodies in size, structure and binding pattern and, importantly, favorable biophysical properties such as exceptional stability. This together with high-yield prokaryotic production renders DARPins promising candidates for microbicide development.
Highlights
Designed Ankyrin Repeat proteins (DARPins) are a novel type of binding protein scaffold designed as antibody alternative for biomedical applications
Gp120 proved a challenging target for selection of DARPin binders against gp120
By combining different selection strategies we were able to derive a variety of gp120-specific DARPin molecules, including some with unique HIV entry blocking activity
Summary
Designed Ankyrin Repeat proteins (DARPins) are a novel type of binding protein scaffold designed as antibody alternative for biomedical applications. DARPins share many properties with antibodies, most noteworthy a high target specificity and affinity, but they differ from antibodies in size, structure and binding pattern and, importantly, favorable biophysical properties such as exceptional stability. This together with high-yield prokaryotic production renders DARPins promising candidates for microbicide development
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