Abstract
Objective To evaluate the confocal and histological microscopic features of corneal interface in rabbit after auto lamellar keratoplasty. Methods Auto lamellar keratoplasty was performed on 20 rabbit eyes. Confocal examination of lamellar interface was performed 1 week, 2 weeks, 1 month and 3 months postoperatively. Five corneal specimens were studied by immunohistochemistry using monoclonal antibodies against CD34 and α -SMA on each time point. Results Confocal microscopy revealed numerous highly reflective keratocytes at the interface 2 weeks postoperatively. Keratocyte density and extracellular matrix reflectivity peaked 1 month postoperatively. The lamellar interface showed positive staining for α -SMA 2 weeks and 1 month postoperatively, while the staining for CD34 weakened. Conclusions Substantial keratocyte activation and corneal opacity occur from 2 weeks to 1 month after auto lamellar keratoplasty. The keratocyte transform into myofibroblast, which may account for the increased corneal refloctivity. Key words: Keratocyte; Myofibroblast; Lamellar keratoplasty; Confocal
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
