Abstract
A versatile approach for absorption spectroscopy on the micron scale is presented allowing label-free characterization of as few as 10∧7 molecules in a pico-liter volume. Our setup employs a confocal microscope with spatial resolution at the cellular level (1-2 microns) over a wide spectral range from the near-infrared to the ultraviolet. Spectra of amino acid, proteins, and DNA bases in solution are acquired on a timescale of seconds with spectral resolution of 0.3 nm. We analyze samples in nanoliter quantities containing a few concentrations of two different dyes. In combinations with factor analysis we can reconstruct the spectra as a superposition of the individual dyes. We explore applications to spatially inhomogeneous samples and single cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
