Abstract
A versatile approach for absorption spectroscopy on the micron scale is presented allowing label-free characterization of as few as 10∧7 molecules in a pico-liter volume. Our setup employs a confocal microscope with spatial resolution at the cellular level (1-2 microns) over a wide spectral range from the near-infrared to the ultraviolet. Spectra of amino acid, proteins, and DNA bases in solution are acquired on a timescale of seconds with spectral resolution of 0.3 nm. We analyze samples in nanoliter quantities containing a few concentrations of two different dyes. In combinations with factor analysis we can reconstruct the spectra as a superposition of the individual dyes. We explore applications to spatially inhomogeneous samples and single cells.
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