Abstract
<p>Tuntun angin is one of important floodplain plants in and around Kajuik Lake located in Riau Province, Indonesia. Morphological identification shows that the scientific name of this plant is <em>Elaeocarpus floribundus. </em>The study aimed to confirm the taxonomic status of tuntun angin using <em>matK</em> and nuclear intergenic spacer (ITS) sequences. The methods included fresh leaf DNA isolation, polymerase chain reaction, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.06 programs. The results showed that the <em>matK</em> sequence (519 bp) of tuntun angin had highest similarity to <em>E. floribundus</em> <em>matK</em> sequence that was available in GenBank. It was supported by the high max score (937), low E-value (0.0), high identity value (100%), and high query cover (100%). However, the ITS sequence of tuntun angin did not show similarity to <em>E. floribundus </em>ITS sequence because there was no database of the sequence in GenBank. This study was able to confirm the taxonomic status of tuntun angin as <em>E. floribundus</em> using <em>matK</em> sequence and also showed that morphological and molecular identification techniques were complementary to each other. Moreover, this study enriched the DNA sequence database of <em>E. floribundus </em>in GenBank which will be useful for this species’ molecular identification.</p><p><strong>How to Cite</strong></p><p>Roslim, D. I., Khumairoh, S. &amp; Herman, H. (2016). Confirmation of Tuntun Angin (<em>Elaeocarpus floribundus</em>) Taxonomic Status Using <em>matK</em> and ITS Sequences. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 393-400. </p>
Highlights
Riau Province has many floodplain ecosystems which contain genetic diversity of the endemic flora and fauna
The genetic distance between tuntun angin and E. floribundus was 0.0 and it means that they are very similar based on the maturase K (matK) sequence
Phylogenetic analysis showed that tuntun angin was clustered in one group and had closer relationship with E. floribundus rather than others
Summary
Riau Province has many floodplain ecosystems which contain genetic diversity of the endemic flora and fauna. Various plants – suggested endemic to Riau – grow in Kajuik Lake, such as rattan (Calamus sp), renghas (Gluta sp), pandan (Pandanus sp), durik-durik (Syzygium sp), and tuntun angin (Elaeocarpus floribundus) (Elvyra & Yus, 2012; Roslim, 2016; Roslim et al, 2016a). Such plants play important roles in maintaining the quality and the quantity of river water, resisting erosion, supplying nutrient for fish and other animals in river, and providing sites of spawning and nursery for fish. Others animals like birds, crabs, shrimps, and insects live in floodplain ecosystem (Banner & MacKenzie, 1998; Price & Lovett, 2002)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
