Abstract
The gender of the last captive thylacine (Thylacinus cynocephalus) or Tasmanian tiger has been a point of debate since its death at the Beaumaris Zoo in Hobart, Tasmania on the night of the 7th September 1936. Recent detailed examination of a single frame from the historic motion film footage taken by Dr David Fleay in 1933 has confirmed that the thylacine was male.
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