Confirmation of minor components of less polar neutral and acidic glycolipids in monkey brain tissue

Tamotsu Taketomi,Eiko Sugiyama,Kei-Ichi Uemura,Atsushi Hara,Hiroya Hidaka,Minoru Tozuka,Tetsuo Nakabayashi,Tsutomu Katsuyama

doi:10.1016/s0022-2275(20)31650-3

Tamotsu Taketomi, Eiko Sugiyama + Show 6 more

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https://doi.org/10.1016/s0022-2275(20)31650-3

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Abstract

Crude glycolipids, prepared without alkali treatment in advance, were separated into neutral and acidic glycolipids by DEAE-Sephadex A-25 (acetate form) column chromatography. Each glycolipid was further fractionated by a Silica gel 60-column chromatography. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with delayed ion extraction (DE MALDI-TOF MS) of the intact glycolipid fractions, the less polar neutral glycolipids were found to contain alkali-labile ester cerebrosides and Galβ-1-Diradylglycerols, whereas the less polar acidic glycolipids were found to contain alkali-labile ester sulfatide, HSO3-3Gal-1-Diradylglycerols, and novel alkali-stable plasmalosulfatides and ester or plasmalo HSO3-3Galβ-1-Diradylglycerols as minor components of glycolipids in monkey brain tissue.In conclusion, minor components of less polar neutral and acidic glycolipids in monkey brain tissue were confirmed as ester cerebrosides, Galβ-1-Diradylglycerols, ester sulfatides, HSO3-3Galβ-1-Diradylglycerols, and novel plasmalo-sulfatides and ester or plasmalo HSO3-3Galβ-1-Diradylglycerols by DE MALDI-TOF MS.

Highlights

Crude glycolipids, prepared without alkali treatment in advance, were separated into neutral and acidic glycolipids by DEAE-Sephadex A-25 column chromatography
DEAE-Sephadex A-25 was purchased from Pharmacia-LKB (Sweden)
Silica gel 60 and precoated thin-layer chromatography (TLC) plates were obtained from Merck (Germany). 2,5-Dihydroxybenzoic acid as the matrix for MALDI-TOF MS was obtained from Sigma Chemical (USA)

Summary

Introduction

Crude glycolipids, prepared without alkali treatment in advance, were separated into neutral and acidic glycolipids by DEAE-Sephadex A-25 (acetate form) column chromatography. Each glycolipid was further fractionated by a Silica gel 60-column chromatography. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with delayed ion extraction (DE MALDI-TOF MS) of the intact glycolipid fractions, the less polar neutral glycolipids were found to contain alkali-labile ester cerebrosides and Gal␤-1Diradylglycerols, whereas the less polar acidic glycolipids were found to contain alkali-labile ester sulfatide, HSO33Gal-1-Diradylglycerols, and novel alkali-stable plasmalosulfatides and ester or plasmalo HSO3-3Gal␤-1-Diradylglycerols as minor components of glycolipids in monkey brain tissue. Minor components of less polar neutral and acidic glycolipids in monkey brain tissue were confirmed as ester cerebrosides, Gal␤-1-Diradylglycerols, ester sulfatides, HSO3-3Gal␤-1-Diradylglycerols, and novel plasmalo-sulfatides and ester or plasmalo HSO3-3Gal␤-1Diradylglycerols by DE MALDI-TOF MS.—Taketomi, T., E. Confirmation of minor components of less polar neutral and acidic glycolipids in monkey brain tissue.

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