Confirmation of isolates of Listeria by conventional and real-time PCR.

Abstract

Polymerase chain reaction (PCR) is an invaluable diagnostic technique in microbiology for rapid and specific detection and confirmation of microbial isolates from food and the environment. PCR is a simple, sensitive, specific, and reproducible assay and can be performed in conventional or in real-time formats. Here, we describe the application of real-time and conventional PCR-based methods for confirmation of presumptive Listeria isolates.